Sanofi Group Scientist- Protein Sciences, Purification in San Diego, California
As a member of the scientific research team, located in La Jolla, CA the Associate Scientist is responsible for developing, leading, and executing all steps of protein purification and scale-up. The Associate Scientist provides principal scientist / senior management with technical proficiency, scientific creativity and professional concepts and procedures to methodically solve problems. He/She is responsible for design and execution of experiments, data analysis and process development/improvement discussions.
Essential duties and responsibilities include the following. Other duties may be assigned.
Design, implement, trouble-shoot, delegate, and execute scientific research and development projects to meet timelines.
Ability to work independently and proactively, and lead research projects as represented by scientific publications, conference presentations and patent filings.
Establish methods for refolding and purification of expressed proteins.
Experimentally develop novel methods to optimize yields from protein purification processes.
Develop standard operating procedures, document protocols and technology transfer.
Prepare and present project updates to ensure timely delivery.
Maintain a high standard for quality control including up-to-date data records for all protein deliveries, including basic understanding of HPLC and mass spectrometry methods for QC.
Troubleshoot challenging technical, experimental, and instrumental issues.
Strong subject matter expertise in downstream purification operations, including chromatography (affinity, ion exchange, HIC, reversed phase and multi-mode), membrane separation, targeted precipitation, protein refolding of inclusion bodies, etc.
Train and lead junior/new team members.
Provide technical guidance and training to co-workers for the use of current and new equipment in the Protein production team.
Responsible for maintenance of AKTA and new equipment within the Production Team.
Consummate ability to work in a highly collaborative, fast-paced, cross-functional environment, with superior oral and written communication skills.
Education / Experience:
Bachelor’s Degree plus minimum 6 years of relevant research experience in an academic or biopharmaceutal setting OR Master’s Degree plus minimum 3 years of relevant research experience in an academic or biopharmaceutical setting OR PhD. No Post doc experience required.
Experience in developing research and scalable processes for protein purification. Hands-on experience with refolding of proteins from inclusion bodies, and filtration strategies (depth filtration, TFF) are a plus.
Hands-on experience with AKTA instrumentation is required. Extensive experience implementing various chromatography modalities (IEX, HIC, multi-mode, reverse phase and SEC), high throughput (HTP) purification strategies, and purification DOE are highly desirable.
Experience in protein characterization (SDS PAGE, Western Blotting, quantification, HPLC, cGE, endotoxin). Understanding of LC/MS methods for QC is a plus.
Regular activities require ability to communicate effectively and rapidly reprioritize to match dynamic and demanding timelines.
Basic understanding of cancer and/or immuno-biology.
Other Working Conditions:
Frequent communication (talking/hearing).
Regular sitting and reaching with arms.
Vision requirements: close, distance, peripheral, color and depth perception, and ability to adjust focus.
Lifting up to 10 lbs.
Computer use: 30% of the time.
Noise conditions: Normal office / lab environment.
May work near moving mechanical parts or in a laboratory setting with chemicals, unpleasant odors, and in areas requiring personal protective equipment.
Interaction with laboratory equipment, samples, supplies, and laboratory personnel may be required whereby appropriate precautions are to be taken per the Company’s Safety and Injury, Illness and Prevention Plans.
Sanofi Inc. and its U.S. affiliates are Equal Opportunity and Affirmative Action employers committed to a culturally diverse workforce. All qualified applicants will receive consideration for employment without regard to race; color; creed; religion; national origin; age; ancestry; nationality; marital, domestic partnership or civil union status; sex, gender, gender identity or expression; affectional or sexual orientation; disability; veteran or military status or liability for military status; domestic violence victim status; atypical cellular or blood trait; genetic information (including the refusal to submit to genetic testing) or any other characteristic protected by law.
At Sanofi diversity and inclusion is foundational to how we operate and embedded in our Core Values. We recognize to truly tap into the richness diversity brings we must lead with inclusion and have a workplace where those differences can thrive and be leveraged to empower the lives of our colleagues, patients and customers. We respect and celebrate the diversity of our people, their backgrounds and experiences and provide equal opportunity for all.